Food Safety Intervention Technologies Research Unit

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Photo of Bill Fett Dr. William F. Fett
Research Microbiologist, Lead Scientist

Voice: 215-233-6418
Fax:  215-233-6406
e-mail:  wfett@errc.ars.usda.gov

New Technologies for Decontaminating Sprouting Seeds and Produce with Easily Damaged Surfaces
 

Senior Scientists

Ching-Hsing Liao,  215-233-6471
cliao@errc.ars.usda.gov

Anabelle Matos,  215-836-3782
amatos@errc.ars.usda.gov

Kathleen T. Rajkowski,  215-233-6440
krajkowski@errc.ars.usda.gov

 

Support Staff

Lee Chau,  215-233-6452
lchau@errc.ars.usda.gov

Kymbrilee Snipes, 215-836-3786
ksnipes@errc.ars.usda.gov

Anita Parameswaran,  215-233-6563
aparameswaran@errc.ars.usda.gov

Jennifer Cassidy, 215-233-6460
jcassidy@errc.ars.usda.gov

 

 

 

  Project #1935-41420-006

Objectives:

The overall objective of the project is to develop new and/or improved methods for eliminating human pathogens from fruits and vegetables, especially sprouting seed and various types of sprouts, melons, and produce with easily damageable surfaces.  Research will primarily focus on developing procedures for produce produced regionally.  New intervention technologies are to be practical and cost effective.  Specific objectives are 1) identify new, more effective chemical treatments to eliminate bacterial human pathogens from sprouting seed and fresh-cut produce, 2) determine the effectiveness of alternative intervention technologies including competitive exclusion and physical treatments, and 3) identify endogenous and exogenous factors which affect the survival, growth and interaction of resident microflora including spoilage bacteria and human pathogens.  As new technologies for decontaminating produce are devised they will be patented (if appropriate) and the technology transferred to the produce production, packing and processing industries.

Major Equipment:

  • Ultracentrifuge (Beckman)
  • Superspeed Centrifuge (Sorvall)
  • Temperature controlled microfuge (eppendorf)
  • Gene Amp PCR System 9700 (PE Applied Biosystems)
  • Modified atmosphere packaging system (Multivac)
  • Horizontal isoelectric focusing apparatus (LKB Multiphor)
  • Ultrafiltration apparatus (Centricon)
  • Electroporation apparatus (BioRad)
  • Microbial ID system (Biolog)
  • BIOFLOW 3000 batch/continuous bioreactor (New Brunswick)
  • Biosafety cabinets (Type II A/B3)
  • Microbiological incubators and incubator/shakers
  • Spectrophotometers
  • Automated plate counter
  • Phase contrast microscopes
  • Stereo microscope (Zeiss)
  • Spiral plater (Autoplate 4000, Spiral Biotech)
  • Stomachers (Seward)
  • Freeze-drier (FTS Systems)

Annual Reports

 

Updated: September 12, 2005